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Addgene

pLV-NT-3-mCherry
(Plasmid #190201)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190201 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti-MP2
  • Backbone manufacturer
    The backbone is described the Naldini group in Dull et al., 1998 (JVI) and was modified by Anthony Oliva and Jia Pingping at the
  • Backbone size w/o insert (bp) 6757
  • Total vector size (bp) 8276
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grown in low sodium LB, 5 grams
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NT-3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1519
  • Mutation
    The codon were optimized for better expression
  • GenBank ID
    NP_001157506
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI, BamHI, XhoI (not destroyed)
  • 3′ cloning site NheI, , XbaI, SalI, EcoRV (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-NT-3-mCherry was a gift from Pantelis Tsoulfas (Addgene plasmid # 190201 ; http://n2t.net/addgene:190201 ; RRID:Addgene_190201)
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