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pAAV-MPRA-MluI-SpeI-EcoRI
(Plasmid #190196)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190196 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-hSyn-EGFP
  • Backbone size (bp) 4061
  • Modifications to backbone
    We digested pAAV-hSyn-EGFP using MluI-HF and EcoR1-HF to remove eGFP from the original backbone, and ligated in an oligo that contains the sequences for MluI, SpeI, and EcoRI restriction sites.
  • Vector type
    Mammalian Expression, AAV ; massively parallel reporter assay (MPRA)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Use Endura Electrocompetent Cells (Lucigen, cat#60242-1). Grow 10 hours at 30C.
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Intended for MPRA libraries.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-MPRA-MluI-SpeI-EcoRI was a gift from Hyejung Won (Addgene plasmid # 190196 ; http://n2t.net/addgene:190196 ; RRID:Addgene_190196)

  • For your References section:

    Systematic investigation of allelic regulatory activity of schizophrenia-associated common variants. McAfee JC, Lee S, Lee J, Bell JL, Krupa O, Davis J, Insigne K, Bond ML, Zhao N, Boyle AP, Phanstiel DH, Love MI, Stein JL, Ruzicka WB, Davila-Velderrain J, Kosuri S, Won H. Cell Genom. 2023 Sep 15;3(10):100404. doi: 10.1016/j.xgen.2023.100404. eCollection 2023 Oct 11. 10.1016/j.xgen.2023.100404 PubMed 37868037
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