pAAV-MPRA-MluI-SpeI-EcoRI
(Plasmid #190196)

• Purpose: (Empty Backbone) Empty vector for inserting MPRA libraries into AAV
• Depositing Lab: Hyejung Won
• Publication: McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. doi: 10.1016/j.xgen.2023.100404. eCollection 2023 Oct 11.

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Backbone

• Vector backbone: pAAV-hSyn-EGFP
• Backbone size (bp): 4061
• Modifications to backbone: We digested pAAV-hSyn-EGFP using MluI-HF and EcoR1-HF to remove eGFP from the original backbone, and ligated in an oligo that contains the sequences for MluI, SpeI, and EcoRI restriction sites.
• Vector type: Mammalian Expression, AAV ; massively parallel reporter assay (MPRA)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Growth instructions: Use Endura Electrocompetent Cells (Lucigen, cat#60242-1). Grow 10 hours at 30C.
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• Supplemental Documents:
  • Won_L6R_1_MPRA-pAAV-MluI-SpeI-EcoR1_pLann.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Intended for MPRA libraries.