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pET-Duet1-His-TEV-mCherry-OPTN WT
(Plasmid #190191)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190191 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET Duet1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5420
  • Total vector size (bp) 7871
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    optineurin
  • Alt name
    NRP
  • Alt name
    FIP2
  • Species
    H. sapiens (human)
  • GenBank ID
    NC_000010.11
  • Entrez Gene
    OPTN (a.k.a. ALS12, FIP2, GLC1E, HIP7, HYPL, NRP, TFIIIA-INTP)
  • Tags / Fusion Proteins
    • 6xHis (N terminal on insert)
    • TEV (N terminal on insert)
    • mCherry (N terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-Duet1-His-TEV-mCherry-OPTN WT was a gift from Sascha Martens (Addgene plasmid # 190191 ; http://n2t.net/addgene:190191 ; RRID:Addgene_190191)

  • For your References section:

    p62 filaments capture and present ubiquitinated cargos for autophagy. Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S. EMBO J. 2018 Mar 1;37(5). pii: embj.201798308. doi: 10.15252/embj.201798308. Epub 2018 Jan 17. 10.15252/embj.201798308 PubMed 29343546
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