p47 PX(1-121)/p40phox(136-339)
(Plasmid
#19018)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19018 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28a
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5369
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep40phox/p47phox
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Alt nameNADPH oxidase subunit
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Alt nameNCF1
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Alt nameNCF4
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SpeciesH. sapiens (human)
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Insert Size (bp)971
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Mutationinsert containing amino acids 1-121 of p47phox fused to amino acids 136-339 of p40phox
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GenBank IDX77094
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Entrez GeneNCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
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Entrez GeneNCF1 (a.k.a. CGD1, NCF1A, NOXO2, SH3PXD1A, p47phox)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p47 PX(1-121)/p40phox(136-339) was a gift from Michael Yaffe (Addgene plasmid # 19018 ; http://n2t.net/addgene:19018 ; RRID:Addgene_19018) -
For your References section:
Phosphatidylinositol 3-phosphate-dependent and -independent functions of p40phox in activation of the neutrophil NADPH oxidase. Bissonnette SA, Glazier CM, Stewart MQ, Brown GE, Ellson CD, Yaffe MB. J Biol Chem. 2008 Jan 25. 283(4):2108-19. 10.1074/jbc.M706639200 PubMed 18029359