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Addgene

pXJ40-GFP-iBox-PAK4cat
(Plasmid #190179)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190179 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pXJ40
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GFP-iBox-PAK4cat
  • Alt name
    INKA-box-PAK4 catalytic domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1791
  • Promoter CMV
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ATGAGTAAAGGAGAAGAACTTTTCAC
  • 3′ sequencing primer TCATCTGGTGCGGTTCTG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pXJ40-GFP-iBox-PAK4cat was a gift from Bianxiao Cui (Addgene plasmid # 190179 ; http://n2t.net/addgene:190179 ; RRID:Addgene_190179)
  • For your References section:

    Engineering a Genetically Encoded Magnetic Protein Crystal. Li TL, Wang Z, You H, Ong Q, Varanasi VJ, Dong M, Lu B, Pasca SP, Cui B. Nano Lett. 2019 Oct 9;19(10):6955-6963. doi: 10.1021/acs.nanolett.9b02266. Epub 2019 Sep 25. 10.1021/acs.nanolett.9b02266 PubMed 31552740