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EB3N-VVDfast-Halo-iLID
(Plasmid #190166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pB80
  • Backbone manufacturer
    Lukas Kapitein lab
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 8487
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EB3N-VVDfast-Halo-iLID
  • Alt name
    EB3N (1-189)-VVDfast-Halo-iLID
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2331
  • Mutation
    1-189 aa from EB3
  • GenBank ID
    AB025186.1
  • Tag / Fusion Protein
    • -VVDfast-mClover3-iLID (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggcttctggcgtgtgaccggcgg
  • 3′ sequencing primer cctcccacacctccccctgaacct
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EB3N-VVDfast-Halo-iLID was a gift from Anna Akhmanova (Addgene plasmid # 190166 ; http://n2t.net/addgene:190166 ; RRID:Addgene_190166)

  • For your References section:

    Opto-katanin, an optogenetic tool for localized, microtubule disassembly. Meiring JCM, Grigoriev I, Nijenhuis W, Kapitein LC, Akhmanova A. Curr Biol. 2022 Nov 7;32(21):4660-4674.e6. doi: 10.1016/j.cub.2022.09.010. Epub 2022 Sep 28. 10.1016/j.cub.2022.09.010 PubMed 36174574
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