pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
(Plasmid #190163)

• Purpose: Microglia-targeted transgene expression.
• Depositing Lab: Hirokazu Hirai
• Publication: Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi: 10.1038/s42003-022-04200-3.

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Backbone

• Vector backbone: pAAV
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: mIba1 promoter, 1.7-kb, microglia-specific promoter
• Insert Size (bp): 1678

Gene/Insert 2

• Gene/Insert name: miR-9-Tx4, 4 repeated miRNA target sites for miR-9
• Insert Size (bp): 102

Gene/Insert 3

• Gene/Insert name: miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
• Insert Size (bp): 102

Resource Information

• Supplemental Documents:
  • pAAV(fITR) mIba1(1700) GFP WPRE miR-9-Tx4 miR-129-2-3p.Tx4 SV40pA_Seq_Confirm.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid concerns patent WO2021177116 - MICROGLIAL SELECTIVE GENE EXPRESSION VECTOR.
https://patentscope2.wipo.int/search/en/detail.jsf?docId=WO2021177116

The depositor has independently confirmed that this plasmid can be packaged despite deletions in ITRs.