Skip to main content
Addgene

pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA
(Plasmid #190163)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190163 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    mIba1 promoter, 1.7-kb, microglia-specific promoter
  • Insert Size (bp)
    1678

Gene/Insert 2

  • Gene/Insert name
    miR-9-Tx4, 4 repeated miRNA target sites for miR-9
  • Insert Size (bp)
    102

Gene/Insert 3

  • Gene/Insert name
    miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
  • Insert Size (bp)
    102

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid concerns patent WO2021177116 - MICROGLIAL SELECTIVE GENE EXPRESSION VECTOR.
https://patentscope2.wipo.int/search/en/detail.jsf?docId=WO2021177116

The depositor has independently confirmed that this plasmid can be packaged despite deletions in ITRs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA was a gift from Hirokazu Hirai (Addgene plasmid # 190163 ; http://n2t.net/addgene:190163 ; RRID:Addgene_190163)

  • For your References section:

    Development of microglia-targeting adeno-associated viral vectors as tools to study microglial behavior in vivo. Okada Y, Hosoi N, Matsuzaki Y, Fukai Y, Hiraga A, Nakai J, Nitta K, Shinohara Y, Konno A, Hirai H. Commun Biol. 2022 Nov 11;5(1):1224. doi: 10.1038/s42003-022-04200-3. 10.1038/s42003-022-04200-3 PubMed 36369525
Related items:
Commonly requested with: