Skip to main content
Addgene

LZ3 dFE
(Plasmid #190144)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190144 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pX165
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LZ3Cas9-T4pol (LZ3 dFE)
  • Species
    Synthetic
  • Insert Size (bp)
    7029
  • Mutation
    Codon Optimized for mammalian cells
  • Tag / Fusion Protein
    • 3xFLAG (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Feng Zhang

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Supplemental GenBank file: LZ3 Cas9 (Plasmid #140561) . Please visit https://doi.org/10.1101/2022.12.05.518807 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZ3 dFE was a gift from Timothy Lu (Addgene plasmid # 190144 ; http://n2t.net/addgene:190144 ; RRID:Addgene_190144)

  • For your References section:

    Frame Editors for Precise, Template-Free Frameshifting. Nakade S, Nakamae K, Tang T-C, Yu D, Sakuma T, Yamamoto T, Lu TK. bioRxiv 2022.12.05.518807 10.1101/2022.12.05.518807
Related items: