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Addgene

pMB2-D
(Plasmid #190125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTU2-D-RFP / pSB1C3
  • Backbone manufacturer
    Paul Freemont Lab / iGem
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    mRFP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Added replications elements for Acinetobacter species into the PstI site; Added Kanamycin resistance cassette into EcoRV site.

NGS assembly indicates that the KanR region is inverted. However, since the plasmid still confers resistance to kanamycin, this mutation is of no concern.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB2-D was a gift from Veronica Godoy-Carter (Addgene plasmid # 190125 ; http://n2t.net/addgene:190125 ; RRID:Addgene_190125)

  • For your References section:

    AspFlex: Molecular Tools to Study Gene Expression and Regulation in Acinetobacter baumannii. Brychcy M, Kokodynski A, Lloyd D, Godoy VG. ACS Synth Biol. 2023 Aug 16. doi: 10.1021/acssynbio.3c00167. 10.1021/acssynbio.3c00167 PubMed 37587063
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