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Addgene

AAV1 for Split-PE, SpCas9 nickase (PKC1001)
(Plasmid #190111)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190111 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nSpCas9
  • Species
    Synthetic
  • Mutation
    H840A
  • Promoter EF1a
  • Tags / Fusion Proteins
    • bpNLS (N terminal on insert)
    • bpNLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV1 for Split-PE, SpCas9 nickase (PKC1001) was a gift from Keith Joung (Addgene plasmid # 190111 ; http://n2t.net/addgene:190111 ; RRID:Addgene_190111)

  • For your References section:

    Engineered CRISPR prime editors with compact, untethered reverse transcriptases. Grunewald J, Miller BR, Szalay RN, Cabeceiras PK, Woodilla CJ, Holtz EJB, Petri K, Joung JK. Nat Biotechnol. 2023 Mar;41(3):337-343. doi: 10.1038/s41587-022-01473-1. Epub 2022 Sep 26. 10.1038/s41587-022-01473-1 PubMed 36163548
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