p40PX-EYFP
(Plasmid #19011)

• Depositing Lab: Michael Yaffe
• Publication: Kanai et al Nat Cell Biol. 2001 Jul . 3(7):675-8.

Backbone

• Vector backbone: EYFP-C1
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: p40phox
• Alt name: NADPH oxidase subunit
• Species: H. sapiens (human)
• Insert Size (bp): 444
• Mutation: aa1-148 (PX domains)
• Entrez Gene: NCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
• Tag / Fusion Protein:
  • EYFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry