ABHD2-3'UTR-Del-2
(Plasmid
#190096)
-
Purposeexamine the activity of ABHD2 3'UTR
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 190096 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepGL3-Control
- Backbone size w/o insert (bp) 5256
- Total vector size (bp) 5818
-
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameABHD2 3' UTR delete-2
-
Alt nameABHD2 3' UTR-Del-2
-
SpeciesH. sapiens (human)
-
Insert Size (bp)562
-
Mutationdeleted the base pairs 7122-7129 refer to NM_007011.8
-
GenBank IDNM_007011.8
-
Entrez GeneABHD2 (a.k.a. HS1-2, LABH2, PHPS1-2)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site FseI (unknown if destroyed)
- 5′ sequencing primer GLp1
- 3′ sequencing primer GLp2 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
ABHD2-3'UTR-Del-2 was a gift from Bowen Liu (Addgene plasmid # 190096 ; http://n2t.net/addgene:190096 ; RRID:Addgene_190096) -
For your References section:
TDP-43 upregulates lipid metabolism modulator ABHD2 to suppress apoptosis in hepatocellular carcinoma. Liu BW, Wang XY, Cao JL, Chen LL, Wang YL, Zhao BQ, Zhou J, Shen ZF. Commun Biol. 2022 Aug 13;5(1):816. doi: 10.1038/s42003-022-03788-w. 10.1038/s42003-022-03788-w PubMed 35963893