pEGFP-TDP-43
(Plasmid #190093)

• Purpose: Expresses TDP-43 in mammalian cells. Note: EGFP and TDP-43 are expressed in different reading frames.
• Depositing Lab: Bowen Liu
• Publication: Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi: 10.1038/s42003-022-03788-w.

Backbone

• Vector backbone: pEGFP-C2
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5945
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAR DNA-Binding Protein 43
• Alt name: TDP-43
• Species: H. sapiens (human)
• Insert Size (bp): 1245
• GenBank ID: NM_007375.4
• Entrez Gene: TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xhol (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C5
• 3′ sequencing primer: EGFP-C3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: EGFP and TDP-43 are expressed in different reading frames.