pET21a-cys-His6-OtUBD
(Plasmid #190091)

• Purpose: Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli
• Depositing Lab: Mark Hochstrasser
• Publication: Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. doi: 10.1371/journal.pbio.3001501. eCollection 2022 Jun.

Backbone

• Vector backbone: pET21a
• Backbone size w/o insert (bp): 5443
• Total vector size (bp): 5734
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: OtUBD
• Alt name: OtDUB(170-264)
• Species: Orientia Tsutsugamushi
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: TCCGGCGTAGAGGATCGAG
• 3′ sequencing primer: CAG CAG CCA ACT CAG CTT CC

Resource Information

• Supplemental Documents:
  • pET_21a_cys_His_OtUBD.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry