pGEX-p40R57Q PX
(Plasmid
#19009)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX4T1
- Backbone size w/o insert (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep40phox
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Alt nameNADPH oxidase subunit
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SpeciesH. sapiens (human)
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Insert Size (bp)444
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Mutationaa1-148 (PX domains) site directed mutagenesis: R57Q
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Entrez GeneNCF4 (a.k.a. CGD3, NCF, P40PHOX, SH3PXD4)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX-5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-p40R57Q PX was a gift from Michael Yaffe (Addgene plasmid # 19009 ; http://n2t.net/addgene:19009 ; RRID:Addgene_19009) -
For your References section:
The PX domains of p47phox and p40phox bind to lipid products of PI(3)K. Kanai F, Liu H, Field SJ, Akbary H, Matsuo T, Brown GE, Cantley LC, Yaffe MB. Nat Cell Biol. 2001 Jul . 3(7):675-8. 10.1038/35083070 PubMed 11433300