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Addgene

pcDNA3_SARS-CoV-Spike[BtKY72 RBD]
(Plasmid #190081)

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Item Catalog # Description Quantity Price (USD)
Plasmid 190081 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3
  • Backbone size w/o insert (bp) 5278
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV S
  • Species
    Synthetic
  • Insert Size (bp)
    3837
  • Entrez Gene
    S (a.k.a. sars2, E2)
  • Promoter CMV
  • Tag / Fusion Protein
    • Rhodopsin C9 (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGCAAATGGGCGGTAGGC
  • 3′ sequencing primer CCTCGACTGTGCCTTCTA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    https://www.addgene.org/145031/

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3_SARS-CoV-Spike[BtKY72 RBD] was a gift from Kenneth Matreyek (Addgene plasmid # 190081 ; http://n2t.net/addgene:190081 ; RRID:Addgene_190081)

  • For your References section:

    Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains. Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA. PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738. 10.1371/journal.pbio.3001738 PubMed 35895696
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