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AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR
(Plasmid #190078)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190078 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Promoterless Bxb1 recombination vector
  • Backbone size w/o insert (bp) 5971
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ACE2
  • Species
    Rhinolophus ferrumequinum
  • Insert Size (bp)
    3972
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Promoter none

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AACGACGGCCAGTGAATTG
  • 3′ sequencing primer CGTTACTGGCCGAAGCCGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human TMPRSS2 co-expressed with ACE2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AttB_ACE2[R.ferrum]-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 190078 ; http://n2t.net/addgene:190078 ; RRID:Addgene_190078)

  • For your References section:

    Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains. Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA. PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738. 10.1371/journal.pbio.3001738 PubMed 35895696
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