AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR
(Plasmid
#190076)
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Purposeexpress human ACE2 with selectable markers iRFP670 and puromycin resistance gene
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190076 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePromoterless Bxb1 recombination vector
- Backbone size w/o insert (bp) 5971
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameACE2
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SpeciesH. sapiens (human)
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Insert Size (bp)2445
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Entrez GeneACE2 (a.k.a. ACEH)
- Promoter none
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer AACGACGGCCAGTGAATTG
- 3′ sequencing primer CGTTACTGGCCGAAGCCGC (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byhttps://www.addgene.org/1786/
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR was a gift from Kenneth Matreyek (Addgene plasmid # 190076 ; http://n2t.net/addgene:190076 ; RRID:Addgene_190076) -
For your References section:
Expanded ACE2 dependencies of diverse SARS-like coronavirus receptor binding domains. Roelle SM, Shukla N, Pham AT, Bruchez AM, Matreyek KA. PLoS Biol. 2022 Jul 27;20(7):e3001738. doi: 10.1371/journal.pbio.3001738. 10.1371/journal.pbio.3001738 PubMed 35895696
