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35S::Cel1SP-GeNL/pCAMBIA1301
(Plasmid #190069)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190069 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCambia1301
  • Backbone manufacturer
    Cambia, Australia
  • Backbone size w/o insert (bp) 11849
  • Total vector size (bp) 14874
  • Vector type
    Plant Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cel1 signal peptide-Green-enhanced Nano-lantern
  • Alt name
    Cel1SP-GeNL
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1260
  • Promoter CaMV 35S

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoT22I (not destroyed)
  • 3′ cloning site SacI (not destroyed)
  • 5′ sequencing primer agcATGCATatggcgcgaaaatccctaattt
  • 3′ sequencing primer CATGGAGCTCTTACGCCAGAATGCGTTCGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    35S::Cel1SP-GeNL/pCAMBIA1301 was a gift from Takeharu Nagai (Addgene plasmid # 190069 ; http://n2t.net/addgene:190069 ; RRID:Addgene_190069)

  • For your References section:

    Application of Green enhanced Nano-lantern as a bioluminescent ratiometric indicator for measurement of Arabidopsis thaliana root apoplastic fluid pH. Tran Q, Osabe K, Entani T, Wazawa T, Hattori M, Nagai T. Plant Cell Environ. 2022 Jul 21. doi: 10.1111/pce.14404. 10.1111/pce.14404 PubMed 35864560
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