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Addgene

BiFC2
(Plasmid #190047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pC2YB
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YemVenus (aa1-172 - TAA)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCGTCCGGAATGTCTAAAGGTGAAGAATTATTCACTGG
  • 3′ sequencing primer CCGACGCGTTTATTCAATGTTGTGTCTAATTTTGAAGTTAGC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BiFC2 was a gift from Patrick Van Dijck (Addgene plasmid # 190047 ; http://n2t.net/addgene:190047 ; RRID:Addgene_190047)

  • For your References section:

    A Bimolecular Fluorescence Complementation Tool for Identification of Protein-Protein Interactions in Candida albicans. Subotic A, Swinnen E, Demuyser L, De Keersmaecker H, Mizuno H, Tournu H, Van Dijck P. G3 (Bethesda). 2017 Oct 5;7(10):3509-3520. doi: 10.1534/g3.117.300149. 10.1534/g3.117.300149 PubMed 28860184
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