pInt-ERCreER
(Plasmid #190040)

• Purpose: This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).
• Depositing Lab: Kayoko Tanaka
• Publication: Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 274087. doi: 10.1242/bio.059056. Epub 2022 Feb 16.

Backbone

• Vector backbone: pBluescript
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 3962
• Total vector size (bp): 12194
• Vector type: Mammalian Expression, CRISPR, TALEN
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ERT2-Cre-ERT2 flanked by AAVS1
• Species: H. sapiens (human); bacteriophage
• Insert Size (bp): 8232

Resource Information

• Supplemental Documents:
  • pInt-ERCreER.gb
• A portion of this plasmid was derived from a plasmid made by: The human AAVS1 sequence of this plasmid is based on pMK243 (Addgene #72835) (Natsume et al. Cell Rep. 2016).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry