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Addgene

pInt-ERCreER
(Plasmid #190040)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190040 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3962
  • Total vector size (bp) 12194
  • Vector type
    Mammalian Expression, CRISPR, TALEN
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERT2-Cre-ERT2 flanked by AAVS1
  • Species
    H. sapiens (human); bacteriophage
  • Insert Size (bp)
    8232

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The human AAVS1 sequence of this plasmid is based on pMK243 (Addgene #72835) (Natsume et al. Cell Rep. 2016).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pInt-ERCreER was a gift from Kayoko Tanaka (Addgene plasmid # 190040 ; http://n2t.net/addgene:190040 ; RRID:Addgene_190040)
  • For your References section:

    Construction of a human hTERT RPE-1 cell line with inducible Cre for editing of endogenous genes. Hindul NL, Jhita A, Oprea DG, Hussain TA, Gonchar O, Campillo MAM, O'Regan L, Kanemaki MT, Fry AM, Hirota K, Tanaka K. Biol Open. 2022 Feb 15;11(2). pii: 274087. doi: 10.1242/bio.059056. Epub 2022 Feb 16. 10.1242/bio.059056 PubMed 35067715