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Addgene

pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP
(Plasmid #190039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FIP200
  • Alt name
    GST-TEVsite-hFIP200 (delta(641-779) & 1363 RDKDLIES 1370 to GSSGGSSG)-MBP
  • Species
    H. sapiens (human)
  • Mutation
    (641-779)deletion & 1363 RDKDLIES 1370 to GSSGGSSG
  • Entrez Gene
    RB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • MBP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (unknown if destroyed)
  • 5′ sequencing primer ctggcaagccacgtttggt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-GST-hFIP200(Delta641-779, 1363-1370Mut)-MBP was a gift from James Hurley (Addgene plasmid # 190039 ; http://n2t.net/addgene:190039 ; RRID:Addgene_190039)

  • For your References section:

    The autophagy adaptor NDP52 and the FIP200 coiled-coil allosterically activate ULK1 complex membrane recruitment. Shi X, Chang C, Yokom AL, Jensen LE, Hurley JH. Elife. 2020 Aug 10;9. pii: 59099. doi: 10.7554/eLife.59099. 10.7554/eLife.59099 PubMed 32773036
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