pTrcHUM
(Plasmid
#19003)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepTrc99A
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Backbone manufacturerpharmacia
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namegamma-humulene synthase
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Alt nameHUM
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SpeciesA. grandis
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
gamma-humulene synthase has been codon optimized for E. coli
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTrcHUM was a gift from Jay Keasling (Addgene plasmid # 19003 ; http://n2t.net/addgene:19003 ; RRID:Addgene_19003) -
For your References section:
Designed divergent evolution of enzyme function. Yoshikuni Y, Ferrin TE, Keasling JD. Nature. 2006 Apr 20. 440(7087):1078-82. 10.1038/nature04607 PubMed 16495946