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nLuc-ΔECD-PTHR1
(Plasmid #190011)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190011 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist CMV Hygro
  • Backbone manufacturer
    Twist Biosciences
  • Backbone size w/o insert (bp) 6600
  • Total vector size (bp) 8472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nLuc-ΔECD-PTHR1
  • Alt name
    nLuc-dECD-PTHR1
  • Alt name
    nLuc-delNT-PTHR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1890
  • Entrez Gene
    PTH1R (a.k.a. EKNS, PFE, PTHR, PTHR1)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Twist Biosciences

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nLuc-ΔECD-PTHR1 was a gift from Sam Gellman (Addgene plasmid # 190011 ; http://n2t.net/addgene:190011 ; RRID:Addgene_190011)

  • For your References section:

    Kinetic and Thermodynamic Insights into Agonist Interactions with the Parathyroid Hormone Receptor-1 from a New NanoBRET Assay. Yu Z, Cary BP, Kim TW, Nguyen KD, Gardella TJ, Gellman SH. ACS Chem Biol. 2022 Nov 18;17(11):3148-3158. doi: 10.1021/acschembio.2c00595. Epub 2022 Oct 25. 10.1021/acschembio.2c00595 PubMed 36282520
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