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Addgene

TTR C10S E63C
(Plasmid #190009)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 190009 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet29
  • Backbone manufacturer
    emdmillipore
  • Backbone size w/o insert (bp) 5371
  • Total vector size (bp) 5758
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TTR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    387
  • Mutation
    C10S, E63C
  • Entrez Gene
    TTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
  • Promoter T7

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid generation in supplementary info of this reference : https://pubs.acs.org/doi/abs/10.1021/cb5004477

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TTR C10S E63C was a gift from Peter Wright (Addgene plasmid # 190009 ; http://n2t.net/addgene:190009 ; RRID:Addgene_190009)

  • For your References section:

    A transthyretin monomer intermediate undergoes local unfolding and transient interaction with oligomers in a kinetically concerted aggregation pathway. Sun X, Ferguson JA, Dyson HJ, Wright PE. J Biol Chem. 2022 Jun 18;298(8):102162. doi: 10.1016/j.jbc.2022.102162. 10.1016/j.jbc.2022.102162 PubMed 35724960
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