TTR C10S S46C
(Plasmid
#190008)
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PurposeExpression of human TTR in E coli
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 190008 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepet29
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Backbone manufactureremdmillipore
- Backbone size w/o insert (bp) 5371
- Total vector size (bp) 5758
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTTR
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SpeciesH. sapiens (human)
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Insert Size (bp)387
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MutationC10S, S46C
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Entrez GeneTTR (a.k.a. ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN)
- Promoter T7
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWe made mutations on the wild type construct received from the Kelly lab. Description of wild type TTR plasmid generation in supplementary info of this reference : https://pubs.acs.org/doi/abs/10.1021/cb5004477
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TTR C10S S46C was a gift from Peter Wright (Addgene plasmid # 190008 ; http://n2t.net/addgene:190008 ; RRID:Addgene_190008) -
For your References section:
A transthyretin monomer intermediate undergoes local unfolding and transient interaction with oligomers in a kinetically concerted aggregation pathway. Sun X, Ferguson JA, Dyson HJ, Wright PE. J Biol Chem. 2022 Jun 18;298(8):102162. doi: 10.1016/j.jbc.2022.102162. 10.1016/j.jbc.2022.102162 PubMed 35724960