MCP-EGFP-PiggyBac
(Plasmid
#190003)
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PurposeExpression of MCP-EGFP in mammalian cells. To clone this plasmid, Addgene plasmid #119908 was used. We replaced the tandem coat proteins and double tagRFP with a single coat protein fused to EGFP.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 190003 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown
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Selectable markersEGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMCP-EGFP
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SpeciesM. musculus (mouse)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCP-EGFP-PiggyBac was a gift from Tineke Lenstra (Addgene plasmid # 190003 ; http://n2t.net/addgene:190003 ; RRID:Addgene_190003) -
For your References section:
Measuring Transcription Dynamics of Individual Genes Inside Living Cells. Brouwer I, de Kort MAC, Lenstra TL. Methods Mol Biol. 2024;2694:235-265. doi: 10.1007/978-1-0716-3377-9_12. 10.1007/978-1-0716-3377-9_12 PubMed 37824008
