pcDNA4/HisMaxB-YAP2-1st&2nd WW mutant
(Plasmid #19000)

• Depositing Lab: Marius Sudol
• Publication: Oka et al J Biol Chem. 2008 Jul 17. ():.

Backbone

• Vector backbone: pcDNA4/HisMaxB
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Yes-kinase associated protein
• Alt name: YAP2, YAP65
• Alt name: YAP2 isoform
• Species: H. sapiens (human)
• Insert Size (bp): 1467
• Mutation: Tryptophan 199 changed to Alanine, and Proline 202 changed to Alanine, and Tryptophan 258 changed to Alanine, and Proline 261 changed to Alanine
• GenBank ID: NM_006106
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Tag / Fusion Protein:
  • His

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHR

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry