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H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)
(Plasmid #189963)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189963 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 6300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    CITED2 (216-269)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    165
  • Mutation
    L243A, E245A, L246A
  • Entrez Gene
    CITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
  • Promoter t7

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    CBP TAZ1 (340-439)
  • Alt name
    CBP CH1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
  • Promoter t7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer t7
  • 3′ sequencing primer t7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439) was a gift from Peter Wright (Addgene plasmid # 189963 ; http://n2t.net/addgene:189963 ; RRID:Addgene_189963)

  • For your References section:

    Multivalency enables unidirectional switch-like competition between intrinsically disordered proteins. Berlow RB, Dyson HJ, Wright PE. Proc Natl Acad Sci U S A. 2022 Jan 18;119(3). pii: 2117338119. doi: 10.1073/pnas.2117338119. 10.1073/pnas.2117338119 PubMed 35012986
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