14x PP7 with loxP-kanMX-loxP
(Plasmid
#189939)
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PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189939 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneunknown
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Vector typeBacterial Expression
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Selectable markerskanMX
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert name14x PP7 with loxP-kanMX-loxP
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- kanMX
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
14x PP7 with loxP-kanMX-loxP was a gift from Tineke Lenstra (Addgene plasmid # 189939 ; http://n2t.net/addgene:189939 ; RRID:Addgene_189939) -
For your References section:
Live-cell imaging reveals the interplay between transcription factors, nucleosomes, and bursting. Donovan BT, Huynh A, Ball DA, Patel HP, Poirier MG, Larson DR, Ferguson ML, Lenstra TL. EMBO J. 2019 Jun 17;38(12). pii: embj.2018100809. doi: 10.15252/embj.2018100809. Epub 2019 May 17. 10.15252/embj.2018100809 PubMed 31101674
