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pET28a-SUMO-MjDim1
(Plasmid #189936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 6194
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rsmA
  • Alt name
    ksgA
  • Species
    Methanocaldococcus jannaschii
  • Insert Size (bp)
    825
  • GenBank ID
    NC_000909.1
  • Entrez Gene
    MJ_RS00455 (a.k.a. MJ_RS00455, MJ0091, MJ_0091)
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • 6x His tag, FLAG tag, SUMO tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bam H1 (not destroyed)
  • 3′ cloning site Xhol 1 (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-SUMO-MjDim1 was a gift from Chuan He (Addgene plasmid # 189936 ; http://n2t.net/addgene:189936 ; RRID:Addgene_189936)

  • For your References section:

    m(6)A RNA modifications are measured at single-base resolution across the mammalian transcriptome. Hu L, Liu S, Peng Y, Ge R, Su R, Senevirathne C, Harada BT, Dai Q, Wei J, Zhang L, Hao Z, Luo L, Wang H, Wang Y, Luo M, Chen M, Chen J, He C. Nat Biotechnol. 2022 Mar 14. pii: 10.1038/s41587-022-01243-z. doi: 10.1038/s41587-022-01243-z. 10.1038/s41587-022-01243-z PubMed 35288668
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