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piggyBAC-H2B mCherry-XL (R125K)
(Plasmid #189926)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189926 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBAC-H2B
  • Backbone size w/o insert (bp) 7000
  • Total vector size (bp) 7800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry-XL (R125K)
  • Species
    Synthetic
  • Insert Size (bp)
    711
  • Mutation
    differs from mCherry by R125K, W143S, I161V, Q163Y and I197R
  • Promoter CMV7
  • Tag / Fusion Protein
    • H2B (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CATCACCAAGTACACCAGC
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggyBAC-H2B mCherry-XL (R125K) was a gift from Ralph Jimenez (Addgene plasmid # 189926 ; http://n2t.net/addgene:189926 ; RRID:Addgene_189926)

  • For your References section:

    Directed Evolution of a Bright Variant of mCherry: Suppression of Nonradiative Decay by Fluorescence Lifetime Selections. Mukherjee S, Manna P, Hung ST, Vietmeyer F, Friis P, Palmer AE, Jimenez R. J Phys Chem B. 2022 Jun 16. doi: 10.1021/acs.jpcb.2c01956. 10.1021/acs.jpcb.2c01956 PubMed 35709514
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