∆C' terminal-DPYSL2-pLJC2
(Plasmid
#189867)
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PurposeExpresses human DPYSL2 (CRMP2) lacking the C' terminus (491-572 aa), in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLJC2
- Backbone size w/o insert (bp) 7438
- Total vector size (bp) 8908
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameΔC-DPYSL2
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Alt nameΔC-CRMP2
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SpeciesH. sapiens (human)
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Insert Size (bp)1470
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Mutationdeleted the C'terminus of DPYSL2 (491-572 aa)
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Tag
/ Fusion Protein
- 3X FLAG (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
∆C' terminal-DPYSL2-pLJC2 was a gift from Yoav Shaul (Addgene plasmid # 189867 ; http://n2t.net/addgene:189867 ; RRID:Addgene_189867) -
For your References section:
DPYSL2 interacts with JAK1 to mediate breast cancer cell migration. Abu Rmaileh A, Solaimuthu B, Khatib A, Lavi S, Tanna M, Hayashi A, Ben Yosef M, Lichtenstein M, Pillar N, Shaul YD. J Cell Biol. 2022 Jul 4;221(7). pii: 213220. doi: 10.1083/jcb.202106078. Epub 2022 May 16. 10.1083/jcb.202106078 PubMed 35575798