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Addgene

pGE144 (MutaEco; aTC-induced)
(Plasmid #189824)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189824 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBAD33
  • Backbone size w/o insert (bp) 5023
  • Total vector size (bp) 6715
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    EcoRNAPα-PmCDA1
  • Alt name
    MutaEco
  • Species
    Synthetic
  • Insert Size (bp)
    1692
  • Promoter tetR/tetA promoter

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer 5'-CAGGAGGAATTCACCCATATGCAGG-3'
  • 3′ sequencing primer pBAD-R (5'-GATTTAATCTGTATCAGG-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGE144 (MutaEco; aTC-induced) was a gift from Seokhee Kim (Addgene plasmid # 189824 ; http://n2t.net/addgene:189824 ; RRID:Addgene_189824)

  • For your References section:

    Development of a genome-targeting mutator for the adaptive evolution of microbial cells. Eom GE, Lee H, Kim S. Nucleic Acids Res. 2022 Apr 22;50(7):e38. doi: 10.1093/nar/gkab1244. 10.1093/nar/gkab1244 PubMed 34928386
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