pHIV-H2BmRFP
(Plasmid #18982)

• Depositing Labs: Bryan Welm, Zena Werb
• Publication: Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102.

Backbone

• Vector backbone: HIV-Zsgreen
• Backbone size w/o insert (bp): 8046
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Growth in Stbl3 bacteria is preferred.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Histone H2B monomeric red fluorescent protein
• Alt name: H2BmRFP
• Alt name: H2B-mRFP
• Species: Discosoma coral
• Insert Size (bp): 1065

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: 5’-TGGAATTTGCCCTTTTTGAG-3’
• 3′ sequencing primer: 5’-AGGAACTGCTTCCTTCACGA-3’

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We obtained H2BmRFP from Sean Megason and Scott Fraser at the California Institute of Technology. The mRFP protein was originally described in Campbell, E.R., et al. PNAS
• Articles Citing this Plasmid:
  • 23 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The HIV-H2BmRFP plasmid was cloned by replacing Zsgreen in HIV-Zsgreen with H2BmRFP to generate a bicistronic lentiviral vector. cDNA can be cloned into the multiple cloning site (HpaI, XbaI, SmaI) in order to coexpress the inserted gene with H2BmRFP. Gerrit Dijkgraaf in Zena Werb's lab cloned this virus.