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Addgene

GB1-His-Tev-ALIX-V (348-702)
(Plasmid #189819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-11a
  • Backbone size w/o insert (bp) 5632
  • Total vector size (bp) 6982
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GB1-His-Tev-ALIX-V (348-702)
  • Alt name
    ALIX-V
  • Alt name
    V Domain of ALIX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1350
  • Entrez Gene
    PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
  • Tag / Fusion Protein
    • GB1-His-Tev (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ALIX UniProt ID: Q8WUM4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GB1-His-Tev-ALIX-V (348-702) was a gift from Lalit Deshmukh (Addgene plasmid # 189819 ; http://n2t.net/addgene:189819 ; RRID:Addgene_189819)

  • For your References section:

    Avidity-Based Method for the Efficient Generation of Monoubiquitinated Recombinant Proteins. Nelson SL, Li Y, Chen Y, Deshmukh L. J Am Chem Soc. 2023 Apr 3. doi: 10.1021/jacs.3c01943. 10.1021/jacs.3c01943 PubMed 37010382
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