E42_gRunx3_gRNA3_dTet_mTurquoise2
(Plasmid
#189806)
-
PurposeRetroviral delivery of guide RNA against mouse Runx3
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 189806 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepQCXIN
-
Vector typeRetroviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namegRunx3_gRNA3
-
gRNA/shRNA sequencegaagcgttgcgcagctcgg
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)19
-
Entrez GeneRunx3 (a.k.a. AML2, Cbfa3, Pebp2a3, Rx3)
Cloning Information
- Cloning method Unknown
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
E42_gRunx3_gRNA3_dTet_mTurquoise2 was a gift from Ellen Rothenberg (Addgene plasmid # 189806 ; http://n2t.net/addgene:189806 ; RRID:Addgene_189806) -
For your References section:
Runx1 and Runx3 drive progenitor to T-lineage transcriptome conversion in mouse T cell commitment via dynamic genomic site switching. Shin B, Hosokawa H, Romero-Wolf M, Zhou W, Masuhara K, Tobin VR, Levanon D, Groner Y, Rothenberg EV. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2019655118. doi: 10.1073/pnas.2019655118. 10.1073/pnas.2019655118 PubMed 33479171