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E42_gRunx1_gRNA2_dTet_NGFR
(Plasmid #189801)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189801 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIN
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    gRunx1_gRNA2
  • gRNA/shRNA sequence
    agccccggcaagatgagcg
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    19
  • Entrez Gene
    Runx1 (a.k.a. AML1, CBF-alpha-2, Cbfa2, Pebp2a2, Pebpa2b)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E42_gRunx1_gRNA2_dTet_NGFR was a gift from Ellen Rothenberg (Addgene plasmid # 189801 ; http://n2t.net/addgene:189801 ; RRID:Addgene_189801)

  • For your References section:

    Runx1 and Runx3 drive progenitor to T-lineage transcriptome conversion in mouse T cell commitment via dynamic genomic site switching. Shin B, Hosokawa H, Romero-Wolf M, Zhou W, Masuhara K, Tobin VR, Levanon D, Groner Y, Rothenberg EV. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4). pii: 2019655118. doi: 10.1073/pnas.2019655118. 10.1073/pnas.2019655118 PubMed 33479171
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