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Addgene

E42_control_NGFR
(Plasmid #189800)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189800 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQCXIN
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Luciferase gRNA
  • gRNA/shRNA sequence
    ggcatttcgcagcctaccg
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    19

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E42_control_NGFR was a gift from Ellen Rothenberg (Addgene plasmid # 189800 ; http://n2t.net/addgene:189800 ; RRID:Addgene_189800)

  • For your References section:

    Bcl11b sets pro-T cell fate by site-specific cofactor recruitment and by repressing Id2 and Zbtb16. Hosokawa H, Romero-Wolf M, Yui MA, Ungerback J, Quiloan MLG, Matsumoto M, Nakayama KI, Tanaka T, Rothenberg EV. Nat Immunol. 2018 Dec;19(12):1427-1440. doi: 10.1038/s41590-018-0238-4. Epub 2018 Oct 30. 10.1038/s41590-018-0238-4 PubMed 30374131
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