p70 delta2-46 delta CT104
(Plasmid
#1898)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 1898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePMT2
- Backbone size w/o insert (bp) 5100
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namep70 alpha1 S6 kinase
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Alt namep70 S6 kinase
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1300
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Mutationdelta2-46, deltaCT104 (deletion of aa 2-46 and amino acids after residue 421)
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Entrez GeneRps6kb1 (a.k.a. p70 S6K-alpha)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p70 delta2-46 delta CT104 was a gift from Joseph Avruch (Addgene plasmid # 1898 ; http://n2t.net/addgene:1898 ; RRID:Addgene_1898) -
For your References section:
Multiple independent inputs are required for activation of the p70 S6 kinase. Weng QP, Andrabi K, Kozlowski MT, Grove JR, Avruch J. Mol Cell Biol 1995 May;15(5):2333-40. PubMed 7739516