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Addgene

E42_gControl_dTet_IRES_mTurquoise2
(Plasmid #189799)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189799 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pQCXIN
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Luciferase gRNA
  • gRNA/shRNA sequence
    ggcatttcgcagcctaccg
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    19

Cloning Information

  • Cloning method Gateway Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    E42_gControl_dTet_IRES_mTurquoise2 was a gift from Ellen Rothenberg (Addgene plasmid # 189799 ; http://n2t.net/addgene:189799 ; RRID:Addgene_189799)
  • For your References section:

    Transcription Factor PU.1 Represses and Activates Gene Expression in Early T Cells by Redirecting Partner Transcription Factor Binding. Hosokawa H, Ungerback J, Wang X, Matsumoto M, Nakayama KI, Cohen SM, Tanaka T, Rothenberg EV. Immunity. 2018 Jun 19;48(6):1119-1134.e7. doi: 10.1016/j.immuni.2018.04.024. 10.1016/j.immuni.2018.04.024 PubMed 29924977