E42_gControl_dTet_IRES_mTurquoise2
(Plasmid
#189799)
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PurposeRetroviral delivery of control guide RNA
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189799 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepQCXIN
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLuciferase gRNA
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gRNA/shRNA sequenceggcatttcgcagcctaccg
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SpeciesM. musculus (mouse)
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Insert Size (bp)19
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
E42_gControl_dTet_IRES_mTurquoise2 was a gift from Ellen Rothenberg (Addgene plasmid # 189799 ; http://n2t.net/addgene:189799 ; RRID:Addgene_189799) -
For your References section:
Transcription Factor PU.1 Represses and Activates Gene Expression in Early T Cells by Redirecting Partner Transcription Factor Binding. Hosokawa H, Ungerback J, Wang X, Matsumoto M, Nakayama KI, Cohen SM, Tanaka T, Rothenberg EV. Immunity. 2018 Jun 19;48(6):1119-1134.e7. doi: 10.1016/j.immuni.2018.04.024. 10.1016/j.immuni.2018.04.024 PubMed 29924977