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Addgene

pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW)
(Plasmid #189791)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFRT
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    beta-globin
  • Alt name
    HBB
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Entrez Gene
    HBB (a.k.a. CD113t-C, ECYT6, beta-globin)
  • Promoter Ponasterone A inducible

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GAAGAACTCAGACACCATACTGCGgctagcATGGTGCACCTGACTCC
  • 3′ sequencing primer CAAATGTGGTATGGCTGATTATGATCactagtcgtatggttttcgg
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-PonA-BI-Gl-WT-24xMS2-WT-18xPP7 (WW) was a gift from Robert Singer (Addgene plasmid # 189791 ; http://n2t.net/addgene:189791 ; RRID:Addgene_189791)

  • For your References section:

    Cellular variability of nonsense-mediated mRNA decay. Sato H, Singer RH. Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0. 10.1038/s41467-021-27423-0 PubMed 34893608
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