pDx_mScarlet3-NCwt-SYFP2
(Plasmid #189766)

• Purpose: Dual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysis
• Depositing Lab: Dorus Gadella
• Publication: Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi: 10.1038/s41592-023-01809-y. Epub 2023 Mar 27.

Backbone

• Vector backbone: pTriEx-3
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mScarlet3-NCwt-SYFP2
• Species: Synthetic
• Mutation: encodes the expected internal mutations relative to mScarlet, but the N- and C-termini of the original mScarlet
• Promoter: CMV & Rha
• Tag / Fusion Protein:
  • 6xHis (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that mScarlet3-NCwt encodes the expected internal mutations of mScarlet3 relative to mScarlet, but the N- and C-termini of the original mScarlet. See the Supplemental Information for the associated paper for more details.