pAAV-EF1a-loxP-GRAB_DA2m-loxP
(Plasmid
#189751)
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PurposeExcludes sensor expression in Cre-positive neurons
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 189751 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4495
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDopamine sensor GRAB_DA2m
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SpeciesH. sapiens (human)
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Insert Size (bp)1857
- Promoter EF1a
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2021.06.22.449478 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-EF1a-loxP-GRAB_DA2m-loxP was a gift from Yulong Li (Addgene plasmid # 189751 ; http://n2t.net/addgene:189751 ; RRID:Addgene_189751) -
For your References section:
Responses and functions of dopamine in nucleus accumbens core during social behaviors. Dai B, Sun F, Tong X, Ding Y, Kuang A, Osakada T, Li Y, Lin D. Cell Rep. 2022 Aug 23;40(8):111246. doi: 10.1016/j.celrep.2022.111246. 10.1016/j.celrep.2022.111246 PubMed 36001967