pLenti-PGK-EGFP-ALDH1A1-Neo
(Plasmid
#189742)
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PurposeThe construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti-PGK-Neo
- Total vector size (bp) 10433
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameALDH1A1
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SpeciesH. sapiens (human)
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Insert Size (bp)1503
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Entrez GeneALDH1A1 (a.k.a. ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1)
- Promoter PGK
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Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-PGK-EGFP-ALDH1A1-Neo was a gift from Robert Sobol (Addgene plasmid # 189742 ; http://n2t.net/addgene:189742 ; RRID:Addgene_189742) -
For your References section:
A specific inhibitor of ALDH1A3 regulates retinoic acid biosynthesis in glioma stem cells. Li J, Garavaglia S, Ye Z, Moretti A, Belyaeva OV, Beiser A, Ibrahim M, Wilk A, McClellan S, Klyuyeva AV, Goggans KR, Kedishvili NY, Salter EA, Wierzbicki A, Migaud ME, Mullett SJ, Yates NA, Camacho CJ, Rizzi M, Sobol RW. Commun Biol. 2021 Dec 21;4(1):1420. doi: 10.1038/s42003-021-02949-7. 10.1038/s42003-021-02949-7 PubMed 34934174
Map uploaded by the depositor.
