pLenti-PGK-EGFP-ALDH1A1-Neo
(Plasmid #189742)

• Purpose: The construct was used to express GFP tagged human ALDH1A1 in mammalian cells for the analysis of the subcellular location of this protein.
• Depositing Lab: Robert Sobol
• Publication: Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi: 10.1038/s42003-021-02949-7.

Backbone

• Vector backbone: pLenti-PGK-Neo
• Total vector size (bp): 10433
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ALDH1A1
• Species: H. sapiens (human)
• Insert Size (bp): 1503
• Entrez Gene: ALDH1A1 (a.k.a. ALDC, ALDH-E1, ALDH1, ALDH11, HEL-9, HEL-S-53e, HEL12, PUMB1, RALDH1)
• Promoter: PGK
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • pLenti-PGK-EGFP-ALDH1A1-Neo.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry