p2xFlag CMV2 Lats1-PY2 mutant
(Plasmid
#18974)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18974 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep2xFlag CMV2
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNM_004690
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Alt nameLats1, PY2 mutant
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SpeciesH. sapiens (human)
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Insert Size (bp)3393
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MutationPY2 mutant (Tyrosine 559 changed to Alanine)
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GenBank IDNM_004690
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Entrez GeneLATS1 (a.k.a. WARTS, wts)
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Tag
/ Fusion Protein
- 2xFlag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p2xFlag CMV2 Lats1-PY2 mutant was a gift from Marius Sudol (Addgene plasmid # 18974 ; http://n2t.net/addgene:18974 ; RRID:Addgene_18974) -
For your References section:
Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976
Map uploaded by the depositor.
