pPpT4- HTA1prom-TIR1 F74G -FLAG
(Plasmid
#189728)
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PurposeEnables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 189728 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepPpT4
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Backbone manufacturerNäätsaari et al., 2012
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Vector typeYeast Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Bleocin (Zeocin), 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1
- Promoter HTA1
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Tag
/ Fusion Protein
- FLAG (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ctttgtctcaggagatccgc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPpT4- HTA1prom-TIR1 F74G -FLAG was a gift from Anita Emmerstorfer-Augustin (Addgene plasmid # 189728 ; http://n2t.net/addgene:189728 ; RRID:Addgene_189728) -
For your References section:
Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii. Lehmayer L, Bernauer L, Emmerstorfer-Augustin A. iScience. 2022 Aug 6;25(9):104888. doi: 10.1016/j.isci.2022.104888. eCollection 2022 Sep 16. 10.1016/j.isci.2022.104888 PubMed 36043049
