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Addgene

pPpT4- TEF2prom-TIR1F74G-FLAG
(Plasmid #189727)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 189727 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPpT4
  • Backbone manufacturer
    Näätsaari et al., 2012
  • Vector type
    Yeast Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Bleocin (Zeocin), 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OsTIR1
  • Promoter TEF2
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPpT4- TEF2prom-TIR1F74G-FLAG was a gift from Anita Emmerstorfer-Augustin (Addgene plasmid # 189727 ; http://n2t.net/addgene:189727 ; RRID:Addgene_189727)

  • For your References section:

    Applying the auxin-based degron system for the inducible, reversible and complete protein degradation in Komagataella phaffii. Lehmayer L, Bernauer L, Emmerstorfer-Augustin A. iScience. 2022 Aug 6;25(9):104888. doi: 10.1016/j.isci.2022.104888. eCollection 2022 Sep 16. 10.1016/j.isci.2022.104888 PubMed 36043049
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