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Addgene

p2xFlag CMV2 Lats1
(Plasmid #18971)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 18971 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p2xFlag CMV2
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Large tumor suppressor kinase 1
  • Alt name
    Lats1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3393
  • Mutation
    *see comments
  • GenBank ID
    NM_004690
  • Entrez Gene
    LATS1 (a.k.a. WARTS, wts)
  • Promoter CMV
  • Tag / Fusion Protein
    • 2xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*note that a user discovered a S1111L mutation in this insert. The origin of this substitution is unknown, as is its effect on function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2xFlag CMV2 Lats1 was a gift from Marius Sudol (Addgene plasmid # 18971)

  • For your References section:

    Mst2 and Lats kinases regulate apoptotic function of YAP. Oka T, Mazack V, Sudol M. J Biol Chem. 2008 Jul 17. ():. 10.1074/jbc.M804380200 PubMed 18640976
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