pcDNA4-hB90
(Plasmid #18968)

• Depositing Lab: Mariano Viapiano
• Publication: Hu et al J Biol Chem. 2008 Jul 7. ():.

Backbone

• Vector backbone: pcDNA3.1+
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Brevican C-terminus
• Alt name: BCAN
• Alt name: CSPG7
• Alt name: BEHAB
• Species: H. sapiens (human)
• Insert Size (bp): 2200
• Mutation: Human brevican signal peptide aa 1-23 followed by C-terminus aa 401-911. Signal peptide between BamHI-EcoRI, C-terminus between EcoRI-NotI (EcoRI site adds two extra aa)
• Entrez Gene: BCAN (a.k.a. BEHAB, CSPG7)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bam HI (not destroyed)
• 3′ cloning site: Not I (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original clone MGC BC010571 purchased from Invitrogen

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry